PVDF Hydrophobic Blotting Membrane Filter, 0.22um, 100mm x 100mm, 25 pcs
Tisch SKU: BMPVDF22100100

PVDF Blotting Membranes, 0.22 µm, 100 mm x 100 mm, 25/pk - BMPVDF22100100

Tisch Scientific backs every order with friendly, knowledgeable support for questions and quotes. Hydrophobic PVDF membranes bind transferred proteins firmly for sensitive immunodetection. The PVDF membrane suits Western detection and protein sequencing while capturing transferred proteins at high binding capacity and readies for electrophoretic transfer. When a Western blot demands durable, high-capacity capture, the PVDF membrane holds the protein through detection.

Key Characteristics

• Compatible with chemiluminescent, colorimetric, and fluorescent detection
• Well suited to high-molecular-weight proteins and protein sequencing
• Retains bound protein under SDS, urea, and organic-solvent wash conditions
• High protein binding captures and holds transferred proteins for sensitive detection
• Hydrophobic surface activates with a brief methanol step before transfer

Strong, even protein transfer and immobilization set up the sensitive detection your blots depend on. Dependable protein immobilization keeps your Western blot quantitative so low-abundance targets stay visible. Request a quality certificate, lot documentation, or a blanket-order quote from the Tisch Scientific support team.

Bubble Point (psi)

17.4psi (IPA)

Color

White

Dimensions

100mm x 100mm

Extractables

Low

Filtration Area

100cm²

Flow Rate

5ml

Grid (Yes/No)

No

Grid Color

/

Incompatibility

Amyl Propyl/Butyle Acetate, Dimethylsulfoxide (DMSO), Dimethyl Formamide, Pyridine, Ketones

Material

PVDF

Maximum Operating Pressure

14.5psi

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

100-130μm

Pack Count

25/pk

pH Range

1-12pH

Pore Size (µm)

0.22

Sterility

Nonsterile

Sterilization

Autoclave @ 121°C for 30min

Weight

0.08

Wettability

Hydrophobic

• Phosphoprotein Detection - Retains bound protein under stringent washes for phospho-specific antibody immunodetection assays.
• Western Blotting - Captures electrophoretically transferred proteins for chemiluminescent and fluorescent immunodetection.
• Protein Sequencing - Immobilizes proteins on a chemically stable membrane for N-terminal sequencing and downstream analysis.
• Quantitative Western - Supports reproducible, high-capacity protein capture for quantitative fluorescent Western workflows.
• High-MW Protein Transfer - Holds high-molecular-weight proteins through transfer and detection with high binding capacity.
• Stripping and Reprobing - Withstands repeated stripping and reprobing cycles for multi-target analysis on a single membrane.