PVDF Hydrophobic Blotting Membrane Filter, 0.45um, 100mm x 100mm, 25 pcs
Tisch SKU: BMPVDF45100100

PVDF Blotting Membranes, 0.45 µm, 100 mm x 100 mm, 25/pk - BMPVDF45100100

Tisch Scientific customer service specialists focus on laboratory products and can help source the right solution for specific applications. Hydrophobic PVDF membranes bind transferred proteins firmly for sensitive immunodetection. Methanol-activated PVDF readies for electrophoretic transfer and stays stable through harsh wash conditions. For high-molecular-weight proteins and sequencing, PVDF immobilizes the target for reliable detection.

Key Characteristics

• Well suited to high-molecular-weight proteins and protein sequencing
• Compatible with chemiluminescent, colorimetric, and fluorescent detection
• Retains bound protein under SDS, urea, and organic-solvent wash conditions
• Mechanically strong membrane withstands stripping and reprobing cycles
• High protein binding captures and holds transferred proteins for sensitive detection

Strong, even protein transfer and immobilization set up the sensitive detection your blots depend on. Dependable protein immobilization keeps your Western blot quantitative so low-abundance targets stay visible. Contact Tisch Scientific for help selecting the right blotting membrane for your transfer and detection workflow.

Bubble Point (psi)

8.7psi (IPA)

Color

White

Dimensions

100mm x 100mm

Extractables

Low

Filtration Area

100cm²

Flow Rate

20ml

Grid (Yes/No)

No

Grid Color

/

Incompatibility

Amyl Propyl/Butyle Acetate, Dimethylsulfoxide (DMSO), Dimethyl Formamide, Pyridine, Ketones

Material

PVDF

Maximum Operating Pressure

14.5psi

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

100-130μm

Pack Count

25/pk

pH Range

1-12pH

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclave @ 121°C for 30min

Weight

0.08

Wettability

Hydrophobic

• Quantitative Western - Supports reproducible, high-capacity protein capture for quantitative fluorescent Western workflows.
• High-MW Protein Transfer - Holds high-molecular-weight proteins through transfer and detection with high binding capacity.
• Protein Sequencing - Immobilizes proteins on a chemically stable membrane for N-terminal sequencing and downstream analysis.
• Stripping and Reprobing - Withstands repeated stripping and reprobing cycles for multi-target analysis on a single membrane.
• Phosphoprotein Detection - Retains bound protein under stringent washes for phospho-specific antibody immunodetection assays.
• Western Blotting - Captures electrophoretically transferred proteins for chemiluminescent and fluorescent immunodetection.