CloneSaver FTA Card, 96-Well Format, Indicating, 5/Pk
Qiagen SKU: WB120028

Featuring indicating FTA technology in a 96-well configuration, this card provides collection, long-term ambient storage, and purification of plasmid and bacterial artificial chromosome (BAC) DNA from bacterial clones. Apply 5 µL of bacterial culture, resuspended colony, or glycerol stock per sample area, with a maximum of 480 µL total per card across all 96 positions. The FTA matrix lyses cells on contact and binds the released DNA to the card fibers, where it remains stabilized and protected from nucleases, oxidation, UV damage, and microbial and fungal attack. The pink-colored matrix changes to white upon application of liquid samples, allowing easy identification of sample location.

Key FTA Media Characteristics

• Indicating FTA matrix lyses cells on contact and sequesters DNA regardless of glycerol content or growth media.
• Color-change technology shifts from pink to white on sample application to confirm and locate each spot.
• Stabilizes plasmid and BAC DNA for room-temperature storage that lasts for years, eliminating the need for freezer space.
• Protects bound nucleic acids from nucleases, oxidation, UV damage, and microbial and fungal contamination.
• Sample layout prevents cross-contamination between adjacent circles when applied as directed.

Key Construction Characteristics

• 96-well sample format with up to 96 samples stored per card.
• Card measures 9.45 in. x 7.87 in. with a 5 µL maximum volume per sample area and 480 µL maximum total volume per card.
• Includes a sample ID chart and an area for bar-code labeling for fast clone identification and recovery.
• Supplied 5 cards per pack.

DNA stored on the card supports many common downstream workflows, including PCR, transformation by electroporation and heat-shock, rolling circle amplification, and sequencing. A 2 mm punch can be used directly in PCR, where products remain in solution and do not bind to the disk for easy recovery, or the DNA can be eluted for further study. This approach delivers reliable access to archived clones without culture regrowth or separate plasmid purification.

Capacity

96 samples per card

Color

Pink (changes to white on sample application)

Dimensions

9.45 in. x 7.87 in.

Indicating Type

Indicating

Material

FTA

Membrane Color

Pink

Pack Count

5 Cards

Volume Throughput (ml)

5 µL maximum per sample area; 480 µL maximum per card

• Clone Archiving Stores plasmid and BAC DNA from bacterial clones at room temperature for years as a secure alternative to glycerol stocks and freezer storage.
• PCR Template Preparation A washed 2 mm punch serves directly as PCR template, with amplification products remaining in solution for easy recovery.
• Bacterial Transformation Eluted or punch-immobilized plasmid DNA transforms bacteria by either electroporation or heat-shock methods.
• Rolling Circle Amplification Plasmid DNA eluted from a punch can be amplified by rolling circle amplification for downstream analysis.
• DNA Sequencing Archived clones can be sequenced without the need for culture regrowth or separate plasmid purification.
• Clone Library Transport Cards enclosed in a multibarrier pouch with desiccant allow clone libraries to be shipped to collaborators at ambient temperature.