Pura Disc 25mm PES Syringe Filters, 0.2um, 50 Pk
Whatman SKU: 6780-2502

Whatman Puradisc Polyethersulfone (PES) Syringe Filters, 0.2 µm, 25 mm, 50/Pk - 6780-2502

Whatman has built such a strong track record in published methods that laboratories default to citing its name in standardized protocols. A sterilizing-grade syringe filter carries manufacturer bacterial retention testing built into the design, giving analysts documented sterile-grade performance without repeating that testing in-house for the format. PES pairs fast flow with reduced protein and antibody adsorption while trapping bacteria reliably at its sterilizing-grade pore rating and stays stable across the pH 1 to 14 range. Low-extractable polypropylene keeps sample purity intact through the filtration pass while holding its form through high-temperature sample preparation conditions and stands firm against aqueous acids, bases, and alcohols during regular handling. Where bacterial contamination must stay out of the sample, manufacturer-tested retention keeps the instrument and downstream assay protected without a housing to maintain or clean.

Technical Specifications

• Non-ionic surface avoids charge-driven protein binding of positively and negatively charged membrane types
• Aromatic sulfone backbone underlies stability across the full pH range
• Sulfone backbone's hydrolytic stability tolerates repeated autoclave steam sterilization cycles
• Finest available pore ratings achieve aseptic-grade filtration performance
• Ionic extractables from the membrane stay very low

Housing Specifications

• Housing tolerates elevated-temperature aqueous and solvent sample filtration up to about 80°C without softening.
• Burst pressure rating provides a wide safety margin above the rated maximum operating pressure.
• Resists stress cracking from aqueous salt and buffer solutions
• Standardized luer connection geometry provides secure engagement with standard syringe tips

Upstream clarification pays for itself by keeping your columns clear and your analytical day on schedule. Upstream sample clarification keeps your separations clean and reproducible across your HPLC and UHPLC methods. Ask our customer support technicians for chemical compatibility guidance on your specific filtration application.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

3.4 bar (50 psi)

Burst Pressure (psi)

75psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Polypropylene

Diameter

25mm

Filtration Area

4.2cm²

Holdup Volume

<100µl

Material

Polyethersulfone (PES)

Maximum Operating Pressure

75psi

Maximum Operating Temperature (°C)

121°C

Pack Count

50

Pore Size (µm)

0.2

Prefilter (Yes/No)

No

Sterility

Nonsterile

Sterilization

Autoclavable 121°C

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophilic

• Urine Drug Screening - Clarifies protein-poor urine samples for dilute-and-shoot LC-MS/MS toxicology testing without adsorbing target drug analytes.
• PFAS Water Testing - PES filters process aqueous samples for EPA Method 1633A PFAS testing without added fluorinated background.
• Cell Culture - Reduces the risk of mycoplasma contamination in growth media, supporting healthier cell lines and more reliable experimental outcomes.
• Organic Carbon Testing - Clarifies water samples ahead of DOC and UV254 analysis while keeping organic carbon adsorption to the membrane minimal.
• Sample Recovery - Minimizes sample loss in low-protein-binding applications, delivering high yield for precious or rare biological specimens.
• Biological Assays - Protects sensitive enzymatic and antibody-based assays from particulate interference without adsorbing critical reagents.