QIAcard FTA Elute Buffer, 40 ml, 1 Unit
Qiagen SKU: WB120100

Featuring a proprietary elution chemistry formulated for QIAcard FTA Elute formats, this buffer enables improved recovery of nucleic acids from samples archived on FTA Elute cards. The buffer improves elution efficiency from a range of inputs, including purified DNA, blood, and saliva. Stored purified DNA bound to the matrix is eluted in 30 minutes, while nucleic acids from blood or saliva samples elute in less than 40 minutes. Each 40 ml bottle supports roughly 200 preps based on, for example, four 3 mm punches per prep, and is designed for combined treatment with Proteinase K to improve lysis and elution efficiency.

Key Buffer Characteristics

• Simplifies the elution protocol while protecting nucleic acids from degradation.
• Delivers DNA immediately suitable for sequencing and PCR applications, including qPCR, RT-qPCR, and subsequent STR analysis.
• Designed for combined treatment with Proteinase K and QIAshredder for improved lysis and elution.
• Offers tailored protocols for purified nucleic acids or for cells and biological fluids.
• Supports flexible sample scaling across various punch sizes and numbers.

Key Handling and Storage Characteristics

• Supplied as a single 40 ml bottle, one unit per pack.
• Shipped at room temperature (15 to 25 degrees C).
• Should be stored at 2 to 8 degrees C upon arrival and is stable until the expiration date indicated on the product.

This buffer maintains nucleic acid integrity throughout collection, room temperature preservation, and elution, supporting reliable downstream results. Its tailored protocols and Proteinase K compatibility provide consistent recovery across diverse sample types. The result is high-quality eluted DNA ready for amplification without additional purification steps.

Capacity

40 ml

Pack Count

1 unit

• Nucleic Acid Elution Provides improved recovery of nucleic acids from QIAcard FTA Elute formats spotted with purified DNA, blood, or saliva.
• PCR and qPCR Preparation Yields DNA immediately suitable for PCR applications such as qPCR and RT-qPCR without additional purification.
• DNA Sequencing Delivers eluted DNA directly compatible with downstream sequencing workflows.
• STR Analysis Supports subsequent short tandem repeat analysis using standard amplification for forensic and identity testing.
• Room Temperature Sample Archiving Pairs with FTA Elute card storage to enable elution of nucleic acids from long-term, room temperature archived samples.
• Proteinase K Workflows Designed for combined treatment with Proteinase K and QIAshredder to improve lysis and elution efficiency.